Lengauer, Christoph; Lüdecke, H. J.; Wienberg, Johannes; Cremer, Thomas; Horsthemke, B.
Comparative chromosome band mapping in primates byin situ suppression hybridization of band specific DNA microlibraries.
In: Human Evolution, Vol. 6, No. 1: pp. 67-71
A DNA-library established from microdissected bands 8q23 to 8q24.1 of normal human chromosomes 8 (Lüdecke et al., 1989) was used as a probe for chromosomal in situ suppression (CISS-) hybridization to metaphase chromosomes of man and primates including Hylobates lar and Macaca fuscata. Comparative band mapping as first applied in this study shows the specific visualization of a single subchromosomal region in all three species and thus demonstrates that synteny of the bulk sequences of a specific human chromosome subregion has been conserved for more than 20 million years.