Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope

Trofymchuk, Kateryna ; Glembockyte, Viktorija ; Grabenhorst, Lennart ; Steiner, Florian ; Vietz, Carolin ; Close, Cindy ; Pfeiffer, Martina; Richter, Lars ; Schütte, Max L.; Selbach, Florian ; Yaadav, Renukka ; Zähringer, Jonas ; Wei, Qingshan ; Ozcan, Aydogan; Lalkens, Birka; Acuna, Guillermo und Tinnefeld, Philip (11. Februar 2021): Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope. In: Nature Communications, Bd. 12, 950

Abstract

The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.

• Dokumententyp: Zeitschriftenartikel
• EU Funded Grant Agreement Number: 840741
• EU-Projekte: Horizon 2020 > Marie Skłodowska Curie Actions
• Publikationsform: Publisher's Version
• Fakultät: Chemie und Pharmazie > Department Chemie
• Fakultätsübergreifende Einrichtungen: Center for NanoScience (CENS)
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie
• Sprache: Englisch