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Trofymchuk, Kateryna ORCID logoORCID: https://orcid.org/0000-0003-3453-1320; Glembockyte, Viktorija ORCID logoORCID: https://orcid.org/0000-0003-2531-6506; Grabenhorst, Lennart ORCID logoORCID: https://orcid.org/0000-0001-9503-1819; Steiner, Florian ORCID logoORCID: https://orcid.org/0000-0002-9148-5837; Vietz, Carolin ORCID logoORCID: https://orcid.org/0000-0002-8417-4168; Close, Cindy ORCID logoORCID: https://orcid.org/0000-0003-4728-033X; Pfeiffer, Martina; Richter, Lars ORCID logoORCID: https://orcid.org/0000-0003-0819-9248; Schütte, Max L.; Selbach, Florian ORCID logoORCID: https://orcid.org/0000-0003-3870-9069; Yaadav, Renukka ORCID logoORCID: https://orcid.org/0000-0002-2633-1639; Zähringer, Jonas ORCID logoORCID: https://orcid.org/0000-0002-5136-0669; Wei, Qingshan ORCID logoORCID: https://orcid.org/0000-0003-1214-010X; Ozcan, Aydogan; Lalkens, Birka; Acuna, Guillermo ORCID logoORCID: https://orcid.org/0000-0001-8066-2677 and Tinnefeld, Philip ORCID logoORCID: https://orcid.org/0000-0003-4290-7770 (11. February 2021): Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope. In: Nature Communications, Vol. 12, 950 [PDF, 1MB]


The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.

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