ORCID: https://orcid.org/0000-0003-3453-1320; Glembockyte, Viktorija ORCID: https://orcid.org/0000-0003-2531-6506; Grabenhorst, Lennart ORCID: https://orcid.org/0000-0001-9503-1819; Steiner, Florian ORCID: https://orcid.org/0000-0002-9148-5837; Vietz, Carolin ORCID: https://orcid.org/0000-0002-8417-4168; Close, Cindy ORCID: https://orcid.org/0000-0003-4728-033X; Pfeiffer, Martina; Richter, Lars ORCID: https://orcid.org/0000-0003-0819-9248; Schütte, Max L.; Selbach, Florian ORCID: https://orcid.org/0000-0003-3870-9069; Yaadav, Renukka ORCID: https://orcid.org/0000-0002-2633-1639; Zähringer, Jonas ORCID: https://orcid.org/0000-0002-5136-0669; Wei, Qingshan ORCID: https://orcid.org/0000-0003-1214-010X; Ozcan, Aydogan; Lalkens, Birka; Acuna, Guillermo ORCID: https://orcid.org/0000-0001-8066-2677 and Tinnefeld, Philip ORCID: https://orcid.org/0000-0003-4290-7770
(11. February 2021):
Addressable nanoantennas with cleared hotspots for single-molecule detection on a portable smartphone microscope.
In: Nature Communications, Vol. 12, 950
[PDF, 1MB]
Abstract
The advent of highly sensitive photodetectors and the development of photostabilization strategies made detecting the fluorescence of single molecules a routine task in many labs around the world. However, to this day, this process requires cost-intensive optical instruments due to the truly nanoscopic signal of a single emitter. Simplifying single-molecule detection would enable many exciting applications, e.g., in point-of-care diagnostic settings, where costly equipment would be prohibitive. Here, we introduce addressable NanoAntennas with Cleared HOtSpots (NACHOS) that are scaffolded by DNA origami nanostructures and can be specifically tailored for the incorporation of bioassays. Single emitters placed in NACHOS emit up to 461-fold (average of 89 ± 7-fold) brighter enabling their detection with a customary smartphone camera and an 8-US-dollar objective lens. To prove the applicability of our system, we built a portable, battery-powered smartphone microscope and successfully carried out an exemplary single-molecule detection assay for DNA specific to antibiotic-resistant Klebsiella pneumonia on the road.
| Item Type: | Journal article |
|---|---|
| EU Funded Grant Agreement Number: | 840741 |
| EU Projects: | Horizon 2020 > Marie Skłodowska Curie Actions |
| Form of publication: | Publisher's Version |
| Faculties: | Chemistry and Pharmacy > Department of Chemistry |
| Research Centers: | Center for NanoScience (CENS) |
| Subjects: | 500 Science > 540 Chemistry |
| URN: | urn:nbn:de:bvb:19-epub-93550-2 |
| Language: | English |
| Item ID: | 93550 |
| Date Deposited: | 04. Nov 2022, 14:03 |
| Last Modified: | 04. Nov 2022, 14:03 |
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