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Berni, Francesca; Kalfopoulou, Ermioni; Cardells, Ana M. Gimeno; Carboni, Filippo; Es, Daan van der; Romero-Saavedra, Felipe; Laverde, Diana; Miklic, Karmela; Malic, Suzana; Rovis, Tihana L.; Jonjic, Stipan; Ali, Sara; Overkleeft, Herman S.; Hokke, Cornelis H.; Diepen, Angela van; Adamo, Roberto; Jimenez-Barbero, Jesus; Marel, Gijsbert A. van der; Hübner, Johannes and Codee, Jeroen D. C. (2021): Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid. In: ACS Chemical Biology, Vol. 16, No. 8: pp. 1344-1349

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Glycerol phosphate (GroP)-based teichoic acids (TAs) are antigenic cell-wall components found in both enterococcus and staphylococcus species. Their immunogenicity has been explored using both native and synthetic structures, but no details have yet been reported on the structural basis of their interaction with antibodies. This work represents the first case study in which a monoclonal antibody, generated against a synthetic TA, was developed and employed for molecular-level binding analysis using TA microarrays, ELISA, SPR-analyses, and STD-NMR spectroscopy. Our findings show that the number and the chirality of the GroP residues are crucial for interaction and that the sugar appendage contributes to the presentation of the backbone to the binding site of the antibody.

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