ONT-Based Draft Genome Assembly and Annotation of Alternaria atra

Bonthala, Bhawna; Small, Corinn S.; Lutz, Maximilian A.; Graf, Alexander; Krebs, Stefan; Sepulveda, German und Stam, Remco (2021): ONT-Based Draft Genome Assembly and Annotation of Alternaria atra. In: Molecular Plant-Microbe Interactions, Bd. 34, Nr. 7: S. 870-873

Abstract

Species of Alternaria (phylum Ascomycota, family Pleosporaceae) are known as serious plant pathogens, causing major losses on a wide range of crops. Alternaria atra (previously known as Ulocladium atrum) can grow as a saprophyte on many hosts and causes Ulocladium blight on potato. It has been reported that it can also be used as a biocontrol agent against Botrytis cinerea. Here, we present a scaffold-level reference genome assembly for A. atra. The assembly contains 43 scaffolds with a total length of 39.62 Mbp, with scaffold N-50 of 3,893,166 bp, L-50 of 4, and the longest 10 scaffolds containing 89.9% of the assembled data. RNA-sequencing-guided gene prediction using BRAKER resulted in 12,173 protein-coding genes with their functional annotation. This first highquality reference genome assembly and annotation for A. atra can be used as a resource for studying evolution in the highly complicated Alternaria genus and might help in understanding the mechanisms defining its role as pathogen or biocontrol agent.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Tiermedizin > Veterinärwissenschaftliches Department
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0894-0282
• Sprache: Englisch