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Bruggen, Myrthe M. van der; Reesink, Koen D.; Spronck, Paul J. M.; Bitsch, Nicole; Hameleers, Jeroen; Megens, Remco T. A.; Schalkwijk, Casper G.; Delhaas, Tammo und Spronck, Bart (2021): An integrated set-up for ex vivo characterisation of biaxial murine artery biomechanics under pulsatile conditions. In: Scientific Reports, Bd. 11, Nr. 1, 2671 [PDF, 3MB]

Abstract

Ex vivo characterisation of arterial biomechanics enables detailed discrimination of the various cellular and extracellular contributions to arterial stiffness. However, ex vivo biomechanical studies are commonly performed under quasi-static conditions, whereas dynamic biomechanical behaviour (as relevant in vivo) may differ substantially. Hence, we aim to (1) develop an integrated set-up for quasi-static and dynamic biaxial biomechanical testing, (2) quantify set-up reproducibility, and (3) illustrate the differences in measured arterial stiffness between quasi-static and dynamic conditions. Twenty-two mouse carotid arteries were mounted between glass micropipettes and kept fully vasodilated. While recording pressure, axial force (F), and inner diameter, arteries were exposed to (1) quasi-static pressure inflation from 0 to 200 mmHg;(2) 300 bpm dynamic pressure inflation (peaking at 80/120/160 mmHg);and (3) axial stretch (lambda(z)) variation at constant pressures of 10/60/100/140/200 mmHg. Measurements were performed in duplicate. Single-point pulse wave velocities (PWV;Bramwell-Hill) and axial stiffness coefficients (c(ax)=dF/d lambda(z)) were calculated at the in vivo value of lambda(z). Within-subject coefficients of variation were similar to 20%. Dynamic PWVs were consistently higher than quasi-static PWVs (p<0.001);c(ax) increased with increasing pressure. We demonstrated the feasibility of ex vivo biomechanical characterisation of biaxially-loaded murine carotid arteries under pulsatile conditions, and quantified reproducibility allowing for well-powered future study design.

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