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Chai, Ruth Jinfen; Werner, Hendrikje; Li, Peter Yiqing; Lee, Yin Loon; Khaing Thet, Nyein; Solovei, Irina; Tuan, Danh Anh Luu; Sharma, Bhavya; Navasankari, Raju; Maric, Martina; Sim, Lois Yu En; Loh, Ying Jie; Aliwarga, Edita; Cheong, Jason Wen Long; Chojnowski, Alexandre; Autio, Matias Ilmari; Haiyang, Yu; Tan, Kenneth Kian Boon; Keng, Choong Tat; Ng, Shi Ling; Chew, Wei Leong; Ferenczi, Michael; Burke, Brian; Foo, Roger Sik Yin and Stewart, Colin L. (2021): Disrupting the LINC complex by AAV mediated gene transduction prevents progression of Lamin induced cardiomyopathy. In: Nature Communications, Vol. 12, No. 1, 4722 [PDF, 8MB]

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Abstract

Mutations in the LaminA gene are a common cause of monogenic dilated cardiomyopathy. Here we show that mice with a cardiomyocyte-specific Lmna deletion develop cardiac failure and die within 3-4 weeks after inducing the mutation. When the same Lmna mutations are induced in mice genetically deficient in the LINC complex protein SUN1, life is extended to more than one year. Disruption of SUN1's function is also accomplished by transducing and expressing a dominant-negative SUN1 miniprotein in Lmna deficient cardiomyocytes, using the cardiotrophic Adeno Associated Viral Vector 9. The SUN1 miniprotein disrupts binding between the endogenous LINC complex SUN and KASH domains, displacing the cardiomyocyte KASH complexes from the nuclear periphery, resulting in at least a fivefold extension in lifespan. Cardiomyocyte-specific expression of the SUN1 miniprotein prevents cardiomyopathy progression, potentially avoiding the necessity of developing a specific therapeutic tailored to treating each different LMNA cardiomyopathy-inducing mutation of which there are more than 450. Mutations in the LaminA gene are the second most common inherited cause of Dilated Cardiomyopathy, a major form of heart failure. Here the authors show that disruption of the nuclear protein SUN1 in cardiomyocytes, by AAV mediated transduction of a SUN1 inhibitor, significantly suppress cardiomyopathy progression, providing a potential therapeutic route to treat this disease.

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