Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II)

Möritz, Annemarie; Grzeschik, Karl-Heinz; Wingender, Edgar and Fink, Edwin (1993): Organization and sequence of the gene encoding the human acrosin-trypsin inhibitor (HUSI-II). In: Gene, Vol. 123, No. 2: pp. 277-281

Abstract

A complete cDNA encoding the acrosin-trypsin inhibitor, HUSI-II, was used as a probe to isolate genomic clones from a human placenta library. Three clones which cover the entire HUSI-II gene were isolated and characterized. The exon-intron organization of the gene was determined and found to be identical to other known Kazal-type inhibitor-encoding genes. The striking similarity in the amino acid sequences which was found previously in HUSI-II and glycoprotein hormone β-subunits, is neither reflected in codon usage nor in the exon-intron arrangement of the genes. A 1.8-kb segment 5′ of the gene was sequenced. The analysis of this sequence showed that HUSI-II contains a G + C-rich region upstream from the transcription start point (tsp) which fulfills the criteria for a CpG island. Furthermore, in the first intron, a potential glucocorticoid-responsive element was found as a half-palindrome flanked by two CACCC elements. Determination of the tsp by S1 mapping revealed that HUSI-II has multiple tsp. Genomic Southern hybridization was used to show that HUSI-II is a single-copy gene. The localization of the gene to chromosome 4 was determined by hybridization of a 5′ genomic fragment to the DNA of a panel of somatic hybrids between human and rodent cells.

• Item Type: Journal article
• Keywords: Kazal-type inhibitor, acrosin inhibitor, exon-intron mapping, nucleotide sequence analysis, transcription start point, chromosomal localization, glycoprotein hormones, chorionic gonadotropin
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 0378-1119