Abstract
In cattle, maternal recognition of early pregnancy depends on the effects of the embryonic signal interferon (IFN)-tau. IFN-stimulated genes have been upregulated in the maternal liver during early pregnancy. In this study, primary hepatocyte cell culture models were evaluated for their suitability to test Type I IFN effects in vitro. The expression of target genes (interferon-stimulated gene 15 (ISG-15), interferon-induced GTP-binding protein (MX-1), C-X-Cmotif chemokine 10 (CXCL-10), CXCL-5, insulin-like growth factor 1 (IGF-1), IGF binding protein 2 (IGFBP-2)) was measured using reverse transcription-quantitative polymerase chain reaction in hepatocytes from monoculture or in indirect coculture with Kupffer cells (HKCid) on Days 1, 2, 3 and 4 of culture (n = 21 donor cows). Gene expression was also measured on Day 4 after challenging the cultures with recombinant IFN tau, IFN alpha, progesterone (P4), IFN tau + IFN alpha or IFN tau + P4 for 6 h. A significant increase in them RNA expression of target genes in hepatocytes was shown in response to stimulation with IFN tau. The Kupffer cells in coculture did not influence the effects of IFN tau in hepatocytes. In conclusion, primary bovine hepatocyte cultures are suitable for stimulation experiments with Type I IFNs and as an extrauterine model for embryo-maternal communication. The proposed endocrine action of IFN tau in the liver may affect maternal metabolism and immune function in the liver.
| Dokumententyp: | Zeitschriftenartikel |
|---|---|
| Fakultät: | Tiermedizin |
| Themengebiete: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit |
| ISSN: | 1031-3613 |
| Sprache: | Englisch |
| Dokumenten ID: | 97934 |
| Datum der Veröffentlichung auf Open Access LMU: | 05. Jun. 2023, 15:27 |
| Letzte Änderungen: | 17. Okt. 2023, 14:57 |
- Dokument bearbeiten
