Sixty days of head-down tilt bed rest with or without artificial gravity do not affect the neuromuscular secretome

Ganse, Bergita; Bosutti, Alessandra; Drey, Michael und Degens, Hans (2021): Sixty days of head-down tilt bed rest with or without artificial gravity do not affect the neuromuscular secretome. In: Experimental Cell Research, Bd. 399, Nr. 2, 112463

Abstract

Artificial gravity is a potential countermeasure to attenuate effects of weightlessness during long-term spaceflight, including losses of muscle mass and function, possibly to some extent attributable to disturbed neuro-muscular interaction. The 60-day AGBRESA bed-rest study was conducted with 24 participants (16 men, 8 women;33 +/- 9 years;175 +/- 9 cm;74 +/- 10 kg;8 control group, 8 continuous (cAG) and 8 intermittent (iAG) centrifugation) to assess the impact of bed rest with or without daily 30-min continuous/intermittent centrifugation with 1G at the centre of mass. Fasting blood samples were collected before and on day 6, 20, 40 and 57 during 6 degrees head-down tilt bed rest. Concentrations of circulating markers of muscle wasting (GDF-8/myostatin;slow skeletal muscle troponin T;prostaglandin E2), neurotrophic factors (BDNF;GDNF) and C-terminal Agrin Fragment (CAF) were determined by ELISAs. Creatine kinase activity was assessed by colorimetric enzyme assay. Repeated-measures ANOVAs were conducted with TIME as within-subject, and INTERVENTION and SEX as between-subject factors. The analyses revealed no significant effect of bed rest or sex on any of the parameters. Continuous or intermittent artificial gravity is a safe intervention that does not have a negative impact of the neuromuscular secretome.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0014-4827
• Sprache: Englisch