B cell depletion impairs vaccination-induced CD8(+) T cell responses in a type I interferon-dependent manner

Graalmann, Theresa; Borst, Katharina; Manchanda, Himanshu; Vaas, Lea; Bruhn, Matthias; Graalmann, Lukas; Koster, Mario; Verboom, Murielle; Hallensleben, Michael; Guzman, Carlos Alberto; Sutter, Gerd; Schmidt, Reinhold E.; Witte, Torsten und Kalinke, Ulrich (2021): B cell depletion impairs vaccination-induced CD8(+) T cell responses in a type I interferon-dependent manner. In: Annals of the Rheumatic Diseases, Bd. 80, Nr. 12: S. 1537-1544

Abstract

Objectives The monoclonal anti-CD20 antibody rituximab is frequently applied in the treatment of lymphoma as well as autoimmune diseases and confers efficient depletion of recirculating B cells. Correspondingly, B cell-depleted patients barely mount de novo antibody responses during infections or vaccinations. Therefore, efficient immune responses of B cell-depleted patients largely depend on protective T cell responses. Methods CD8(+) T cell expansion was studied in rituximab-treated rheumatoid arthritis (RA) patients and B cell-deficient mice on vaccination/infection with different vaccines/pathogens. Results Rituximab-treated RA patients vaccinated with Influvac showed reduced expansion of influenza-specific CD8(+) T cells when compared with healthy controls. Moreover, B cell-deficient JHT mice infected with mouse-adapted Influenza or modified vaccinia virus Ankara showed less vigorous expansion of virus-specific CD8(+) T cells than wild type mice. Of note, JHT mice do not have an intrinsic impairment of CD8(+) T cell expansion, since infection with vaccinia virus induced similar T cell expansion in JHT and wild type mice. Direct type I interferon receptor signalling of B cells was necessary to induce several chemokines in B cells and to support T cell help by enhancing the expression of MHC-I. Conclusions Depending on the stimulus, B cells can modulate CD8(+) T cell responses. Thus, B cell depletion causes a deficiency of de novo antibody responses and affects the efficacy of cellular response including cytotoxic T cells. The choice of the appropriate vaccine to vaccinate B cell-depleted patients has to be re-evaluated in order to efficiently induce protective CD8(+) T cell responses.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Tiermedizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0003-4967
• Sprache: Englisch