Catching Speedy Gonzales: Driving forces for Protein Film Formation on Silicone Rubber Tubing During Pumping

Deiringer, Natalie; Ruediger, Daniel; Luxbacher, Thomas; Zahler, Stefan und Friess, Wolfgang (2022): Catching Speedy Gonzales: Driving forces for Protein Film Formation on Silicone Rubber Tubing During Pumping. In: Journal of Pharmaceutical Sciences, Bd. 111, Nr. 6: S. 1577-1586

Abstract

Interfacial adsorption is a major concern in the processing of biopharmaceutics as it not only leads to a loss of protein, but also to particle formation. Protein particle formation during peristaltic pumping is linked to interfacial adsorption to the tubing and subsequent tearing of the formed protein film. In the current study, driving forces and rate of the adsorption of a monoclonal antibody to the silicone rubber surface during pumping, as well as particle formation, were studied in different formulations. Particle concentration and size distribution were influenced by the formulation parameters;specifically high ionic strength led to more particles and the build-up of particles larger than 25 mm. Formulation pH and ionic strength had an effect on the total amount of adsorbed protein. Adsorbed protein amounts increased when the Debye length of the protein was decreased, leading to a higher packing density. Atomic force microscopy and streaming potential determination revealed that the irreversible protein film formation on the hydrophobic tubing surface occurs in less than a second. Electrostatic interactions are the dominating factor for the initial adsorption speed. In intimate contact to the silicone rubber surface, hydrophobic interactions govern the protein adsorption. PS20 quickly coats the tubing surface which leads to an increase in hydrophilicity and shielding of electrostatic interactions, thereby efficiently inhibiting protein adsorption. Overall, atomic force microscopy and streaming potential determination possess great potential for the characterization of adsorbed protein films and the adsorption kinetic evaluation in high-speed mode. Protein adsorption to silicone tubing is driven by a combination of electrostatic and hydrophobic interactions which is effectively shielded by PS20. (C) 2022 American Pharmacists Association. Published by Elsevier Inc. All rights reserved.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Chemie und Pharmazie > Department für Pharmazie - Zentrum für Pharmaforschung
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie
• ISSN: 0022-3549
• Sprache: Englisch