Probing cerebellar circuit dysfunction in rodent models of spinocerebellar ataxia by means of in vivo twophoton calcium imaging

Douthwaite, Christopher; Tietje, Christoph; Ye, Xiaoqian und Liebscher, Sabine (2024): Probing cerebellar circuit dysfunction in rodent models of spinocerebellar ataxia by means of in vivo twophoton calcium imaging. In: Star Protocols, Bd. 5, Nr. 1, 102911

Abstract

Purkinje neuron degeneration characterizes spinocerebellar ataxia type 1, yet the comprehension of the impact on the broader cerebellar circuit remains incomplete. We here detail simultaneous in vivo two -photon calcium imaging of diverse neuronal populations in the cerebellar cortex of Sca1 mice while they are navigating a virtual environment. We outline surgical procedures and protocols to chronically record from identical neurons, and we detail data postprocessing and analysis to delineate disease -related alterations in neuronal activity and sensorimotor-driven response properties. For complete details on the use and execution of this protocol, please refer to Pilotto et al.1

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin > Munich Cluster for Systems Neurology (SyNergy)
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 390857198
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 491502892