A macrophage-T cell coculture model for severe tissue injury-induced T cell death

Zhu, Jie; Cao, Jiayu; Liesz, Arthur und Roth, Stefan (2021): A macrophage-T cell coculture model for severe tissue injury-induced T cell death. In: Star Protocols, Bd. 2, Nr. 4, 100983

Abstract

A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macro-phages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflamma-some activation in macrophages.For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin > Munich Cluster for Systems Neurology (SyNergy)
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 390857198