ATUM-FIB microscopy for targeting and multiscale imaging of rare events in mouse cortex

Kislinger, Georg; Gnaegi, Helmut; Kerschensteiner, Martin; Simons, Mikael; Misgeld, Thomas und Schifferer, Martina (2020): ATUM-FIB microscopy for targeting and multiscale imaging of rare events in mouse cortex. In: Star Protocols, Bd. 1, Nr. 3, 100232

Abstract

Here, we describe a detailed workflow for ATUM-FIB microscopy, a hybrid method that combines serial-sectioning scanning electron microscopy (SEM) with focused ion beam SEM (FIB-SEM). This detailed protocol is optimized for mouse cortex samples. The main processing steps include the generation of semi-thick sections from sequentially cured resin blocks using a heated microtomy approach. We demonstrate the different imaging modalities, including serial light and electron microscopy for target recognition and FIB-SEM for isotropic imaging of regions of interest.For complete details on the use and execution of this protocol, please refer to Kislinger et al. (2020).

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin > Munich Cluster for Systems Neurology (SyNergy)
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 390857198