Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation

Lange, Andreas; Medugorac, Ivica ; Ali, Asghar; Kessler, Barbara; Kurome, Mayuko; Zakhartchenko, Valeri; Hammer, Sabine E. ; Hauser, Andreas ; Denner, Joachim ; Dobenecker, Britta; Wess, Gerhard; Tan, Paul L. J.; Garkavenko, Olga; Reichart, Bruno ; Wolf, Eckhard und Kemter, Elisabeth (2024): Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation. In: Xenotransplantation, Bd. 31, Nr. 2

Abstract

One of the prerequisites for successful organ xenotransplantation is a reasonable size match between the porcine organ and the recipient's organ to be replaced. Therefore, the selection of a suitable genetic background of source pigs is important. In this study, we investigated body and organ growth, cardiac function, and genetic diversity of a colony of Auckland Island pigs established at the Center for Innovative Medical Models (CiMM), LMU Munich. Male and female Auckland Island pig kidney cells (selected to be free of porcine endogenous retrovirus C) were imported from New Zealand, and founder animals were established by somatic cell nuclear transfer (SCNT). Morphologically, Auckland Island pigs have smaller body stature compared to many domestic pig breeds, rendering their organ dimensions well-suited for human transplantation. Furthermore, echocardiography assessments of Auckland Island pig hearts indicated normal structure and functioning across various age groups throughout the study. Single nucleotide polymorphism (SNP) analysis revealed higher runs of homozygosity (ROH) in Auckland Island pigs compared to other domestic pig breeds and demonstrated that the entire locus coding the swine leukocyte antigens (SLAs) was homozygous. Based on these findings, Auckland Island pigs represent a promising genetic background for organ xenotransplantation.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Chemie und Pharmazie > GenZentrum
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0908-665X
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 213602983
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 314061271