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Bocchi, Riccardo ORCID logoORCID: https://orcid.org/0000-0002-4105-6807; Thorwirth, Manja ORCID logoORCID: https://orcid.org/0000-0002-7692-546X; Simon-Ebert, Tatiana; Koupourtidou, Christina ORCID logoORCID: https://orcid.org/0000-0002-8352-1498; Clavreul, Solène ORCID logoORCID: https://orcid.org/0000-0001-5384-8425; Kolf, Keegan ORCID logoORCID: https://orcid.org/0009-0003-9896-6101; Della Vecchia, Patrizia ORCID logoORCID: https://orcid.org/0009-0001-5623-4581; Bottes, Sara; Jessberger, Sebastian ORCID logoORCID: https://orcid.org/0000-0002-0056-8275; Zhou, Jiafeng ORCID logoORCID: https://orcid.org/0009-0005-5056-3762; Wani, Gulzar ORCID logoORCID: https://orcid.org/0000-0003-2380-5816; Pilz, Gregor-Alexander ORCID logoORCID: https://orcid.org/0000-0002-6161-4976; Ninkovic, Jovica ORCID logoORCID: https://orcid.org/0000-0002-4381-0041; Buffo, Annalisa ORCID logoORCID: https://orcid.org/0000-0001-7637-0006; Sirko, Swetlana ORCID logoORCID: https://orcid.org/0000-0001-5950-616X; Götz, Magdalena ORCID logoORCID: https://orcid.org/0000-0003-1551-9203 und Fischer-Sternjak, Judith ORCID logoORCID: https://orcid.org/0000-0003-0398-7720 (2025): Astrocyte heterogeneity reveals region-specific astrogenesis in the white matter. In: Nature Neuroscience, Bd. 28: S. 457-469 [PDF, 12MB]

Abstract

Astrocyte heterogeneity has been well explored, but our understanding of white matter (WM) astrocytes and their distinctions from gray matter (GM) astrocytes remains limited. Here, we compared astrocytes from cortical GM and WM/corpus callosum (WM/CC) using single-cell RNA sequencing and spatial transcriptomics of the murine forebrain. The comparison revealed similarities but also significant differences between WM and GM astrocytes, including cytoskeletal and metabolic hallmarks specific to WM astrocytes with molecular properties also shared with human WM astrocytes. When we compared murine astrocytes from two different WM regions, the cortex and cerebellum, we found that they exhibited distinct, region-specific molecular properties, with the cerebellum lacking, for example, a specific cluster of WM astrocytes expressing progenitor and proliferation genes. Functional experiments confirmed astrocyte proliferation in the WM/CC, but not in the cerebellar WM, suggesting that the WM/CC may be a source of continued astrogenesis.

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