DNA Mimic Foldamer Recognition of a Chromosomal Protein

Deepak, Deepak ; Wu, Jiaojiao ; Corvaglia, Valentina ; Allmendinger, Lars ; Scheckenbach, Michael ; Tinnefeld, Philip und Huc, Ivan (2025): DNA Mimic Foldamer Recognition of a Chromosomal Protein. In: Angewandte Chemie International Edition, Bd. 64, Nr. 8, e202422958

Abstract

Helical aromatic oligoamide foldamers bearing anionic side chains that mimic the overall shape and charge surface distribution of DNA were synthesized. Their interactions with chromosomal protein Sac7d, a non-sequence-selective DNA-binder that kinks DNA, were investigated by Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), Circular Dichroism spectroscopy (CD), melting curve analysis, Atomic Force Microscopy (AFM), and Nuclear Magnetic Resonance (NMR), as well as by single crystal X-ray crystallography. The foldamers were shown to bind to Sac7d better than a DNA duplex of comparable length. The interaction is diastereoselective and takes place at the DNA binding site. Crystallography revealed that the DNA mimic foldamers have a binding mode of their own and that they can bind to Sac7d without being kinked.

• Dokumententyp: Zeitschriftenartikel
• EU Funded Grant Agreement Number: ERC-2021-ADG-320892
• EU-Projekte: Horizon Europe > ERC Grants
• Fakultät: Chemie und Pharmazie > Department für Pharmazie - Zentrum für Pharmaforschung
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie
• ISSN: 1433-7851
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 325871075