APOSTEL-R Recommendations for Reporting Retinal Optical Coherence Tomography Studies in Rodents

Oertel, Frederike Cosima ; Cabrera Debuc, Delia; Calabresi, Peter A. ; Chen, Mei ; Cordano, Christian ; Dietrich, Michael; Feltgen, Nicolas; Gramlich, Oliver ; Green, Ari J. ; Groh, Janos ; Huang, Su-Chun ; Knier, Benjamin ; Korn, Thomas ; Leocani, Letizia ; Loewenstein, Anat; Oterendorp, Christian van; Peto, Tunde; Schippling, Sven; Schmetterer, Leopold; Schmitz-Valckenberg, Steffen; Seeliger, Mathias W. ; Shindler, Kenneth S. ; Schuman, Joel S. ; Sindi, Mustafa ; Tufail, Adnan; Wang, Jui-Kai ; Wolf, Sebastian ; You, Yuyi ; Zinkernagel, Martin; Lagrèze, Wolf und Albrecht, Philipp (2025): APOSTEL-R Recommendations for Reporting Retinal Optical Coherence Tomography Studies in Rodents. In: Neurology Neuroimmunology & Neuroinflammation, Bd. 12, Nr. 6, e200489

Abstract

Background and Objectives

Retinal opticalcoherence tomography (OCT)in rodent models has been used to longitudinally image retinalchanges, to define end points for more costly or time-consuming experiments,and to better understand the pathophysiology underlying OCT findings in human diseases. No standardization of rodent OCT reporting currently exists. Here, we aim to establish consensus recommendation for reporting results from retinal OCT studies in rodents.

Methods

Initial recommendations were developed based on the APOSTEL criteria for quantitative OCT reporting in humans by acore team.Using a modified Delphi process,an expert panel ofrodent OCT researchers(N = 31)and the widerscientificcommunity discussed, efined,and voted on these initial recommendations. The list of recommendations was then revised and approved by the expert panel.

Results

The final 7-point checklist includes reporting recommendations regarding the study protocol, OCT device, acquisition settings and modifications, scanning protocol, funduscopic imaging, postacquisition data selection and image data analyses, and qualitative and quantitative results. With a median agreement score of 3 or 4 out of 4, the scientific community agreed with these recommendations. After revisions, the expert panel accepted the final recommendations.

Discussion

The Advised Protocol for OCT Study Terminology and Elements for reporting OCT studies in rodents (APOSTEL-R) originates from an expert consensus. They will provide guidance throughout the experimental process and will contribute to the standardization and quality improvement of preclinical OCT studies.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin > Munich Cluster for Systems Neurology (SyNergy)
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 2332-7812
• Bemerkung: for the IMSVISUAL consortium
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 390857198