Proteasome assembly from 15S precursors involves major conformational changes and recycling of the Pba1-Pba2 chaperone

Kock, Malte; Nunes, Maria M.; Hemann, Matthias; Kube, Sebastian; Dohmen, R. Jürgen; Herzog, Franz; Ramos, Paula C. und Wendler, Petra (2015): Proteasome assembly from 15S precursors involves major conformational changes and recycling of the Pba1-Pba2 chaperone. In: Nature Communications, Bd. 6, 6123

Abstract

The chaperones Ump1 and Pba1-Pba2 promote efficient biogenesis of 20S proteasome core particles from its subunits via 15S intermediates containing alpha and beta subunits, except beta7. Here we elucidate the structural role of these chaperones in late steps of core particle biogenesis using biochemical, electron microscopy, cross-linking and mass spectrometry analyses. In 15S precursor complexes, Ump1 is largely unstructured, lining the inner cavity of the complex along the interface between alpha and beta subunits. The alpha and beta subunits form loosely packed rings with a wider alpha ring opening than in the 20S core particle, allowing for the Pba1-Pba2 heterodimer to be partially embedded in the central alpha ring cavity. During biogenesis, the heterodimer is expelled from the alpha ring by a restructuring event that organizes the beta ring and leads to tightening of the alpha ring opening. In this way, the Pba1-Pba2 chaperone is recycled for a new round of proteasome assembly.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Chemie und Pharmazie > Department Biochemie
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie
• ISSN: 2041-1723
• Sprache: Englisch