Design of Poly-l-Glutamate-Based Complexes for pDNA Delivery

Nino-Pariente, Amaya; Arminan, Ana; Reinhard, Sören; Scholz, Claudia; Wagner, Ernst und Vicent, Maria J. (2017): Design of Poly-l-Glutamate-Based Complexes for pDNA Delivery. In: Macromolecular Bioscience, Vol. 17, No. 10, 1700029

Abstract

Due to the polyanionic nature of DNA, typically cationic or neutral delivery vehicles have been used for gene delivery. As a new approach, this study focuses on the design, development, and validation of nonviral polypeptide-based carriers for oligonucleotide delivery based on a negatively charged poly-l-glutamic acid (PGA) backbone partly derivatized with oligoaminoamide residues. To this end, PGA-derivatives modified with different pentameric succinyl tetraethylene pentamines (Stp(5)) are designed. Optionally, histidines for modulation of endosomal buffer capacity and cysteines for pDNA complex stabilization are included, followed by characterization of biophysical properties and gene transfer efficiency in N2a neuroblastoma or 4T1 breast cancer cells.

• Item Type: Journal article
• Faculties: Chemistry and Pharmacy > Department of Pharmacy
• Research Centers: Center for NanoScience (CENS)
• Subjects: 500 Science > 540 Chemistry; 500 Science > 500 Science
• ISSN: 1616-5187
• Language: English