Logo Logo
Hilfe
Hilfe
Switch Language to English

Hammink, Roel; Eggermont, Loek J.; Zisis, Themistoklis; Tel, Jurjen; Figdor, Carl G.; Rowan, Alan E. und Blank, Kerstin G. (2017): Affinity-Based Purification of Polyisocyanopeptide Bioconjugates. In: Bioconjugate Chemistry, Bd. 28, Nr. 10: S. 2560-2568

Volltext auf 'Open Access LMU' nicht verfügbar.

Abstract

Water-soluble polyisocyanopeptides (PICs) are a new dass of synthetic polymers that mimic natural protein based filaments. Their unique semiflexible properties combined with a length of several hundred nanometers have recently enabled a number of biomedical applications ranging from tissue engineering to cancer immunotherapy. One crucial step toward the further development of PICs for these applications is the efficient and controlled synthesis and purification of PIC-biomolecule conjugates. Considering the large size of PICs and the biomolecules to be conjugated, conjugation reactions do usually not proceed to completion due to steric effects. As a consequence, purification of the reaction mixture is necessary to separate the obtained bioconjugates from unreacted biomolecules. As a direct result of the semiflexible nature of PICs, standard polymer and protein purification methods based on molecular weight have not been successful. Here, we introduce a new affinity-based purification method utilizing biotin as an affinity tag. PICs decorated with a controlled and tunable density of biotin molecules (biotinPlCs) were efficiently bound to and eluted from a monoavidin resin in buffered aqueous solution. Using these biotinPlCs, two different protein conjugates were synthesized, one carrying the enzyme alkaline phosphatase (PhoA) and the other T-cell activating anti-CD3 antibodies. The resulting biotinPlC-protein conjugates were successfully obtained in high purity (>90%) and without any loss of protein activity. The high purity greatly simplifies the analysis of biotinPlC bioconjugates, such as the determination of the average number of biomolecules conjugated per biotinPlC chain. Most importantly, it allows for the direct and straightforward application of the obtained bioconjugates in the desired applications. The new method developed may further be adapted for the purification of other advanced bioconjugates that are difficult to obtain in high purity with the available standard methods.

Dokument bearbeiten Dokument bearbeiten