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Lin, Hsiao-Chun Amy; Dean-Ben, Xose Luis; Reiss, Michael; Schöttle, Verena; Wahl-Schott, Christian A.; Efimov, Igor R.; Razansky, Daniel (2018): Ultrafast Volumetric Optoacoustic Imaging of Whole Isolated Beating Mouse Heart. In: Scientific Reports, Vol. 8, 14132
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Abstract

The Langendorff-perfused heart technique has become the model of choice for multiparametric optical mapping of cardiac function and electrophysiology. However, photon scattering in tissues represents a significant drawback of the optical imaging approach, fundamentally limiting its mapping capacity to the heart surface. This work presents the first implementation of the optoacoustic approach for 4D imaging of the entire beating isolated mouse heart. The method combines optical excitation and acoustic detection to simultaneously render rich optical contrast and high spatio-temporal resolution at centimeter-scale depths. We demonstrate volumetric imaging of deeply located cardiac features, including the interventricular septum, chordae tendineae, and papillary muscles while further tracking the heart beat cycle and the motion of the pulmonary, mitral, and tricuspid valves in real time. The technique possesses a powerful combination between high imaging depth, fast volumetric imaging speed, functional and molecular imaging capacities not available with other imaging modalities currently used in cardiac research.