Self-Healing Dyes - Keeping the Promise?

Isselstein, Michael; Zhang, Lei; Glembockyte, Viktorija; Brix, Oliver; Cosa, Gonzalo; Tinnefeld, Philip und Cordes, Thorben (2020): Self-Healing Dyes - Keeping the Promise? In: Journal of physical chemistry letters, Bd. 11, Nr. 11: S. 4462-4480

Abstract

Self-healing dyes have emerged as a new promising class of fluorescent labels. They consist of two units, a fluorescent dye and a photostabilizer. The latter heals whenever the fluorescent dye is in danger of taking a reaction pathway toward photobleaching. We describe the underlying concepts and summarize the developmental history and state-of-the-art, including latest applications in high-resolution microscopy, live-cell, and single-molecule imaging. We further discuss remaining limitations, which are (i) lower photostabilization of most self-healing dyes when compared to solution additives, (ii) limited mechanistic understanding on the influence of the biochemical environment and molecular oxygen on self-healing, and (iii) the lack of cheap and facile bioconjugation strategies. Finally, we provide ideas on how to further advance self-healing dyes, show new data on redox blinking caused by double-stranded DNA, and highlight forthcoming work on intramolecular photostabilization of fluorescent proteins.

• Dokumententyp: Zeitschriftenartikel
• EU Funded Grant Agreement Number: 638536
• EU-Projekte: Horizon 2020 > ERC Grants > ERC Starting Grant > ERC Grant 638536: SM-IMPORT - Substrate import at work: single-molecule studies of ABC transporters
• Fakultät: Biologie > Department Biologie I
• Themengebiete: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
• Sprache: Englisch