ORCID: https://orcid.org/0000-0002-8598-5499
(2020):
Self-Healing Dyes - Keeping the Promise?
In: Journal of physical chemistry letters, Vol. 11, No. 11: pp. 4462-4480
[PDF, 12MB]

Abstract
Self-healing dyes have emerged as a new promising class of fluorescent labels. They consist of two units, a fluorescent dye and a photostabilizer. The latter heals whenever the fluorescent dye is in danger of taking a reaction pathway toward photobleaching. We describe the underlying concepts and summarize the developmental history and state-of-the-art, including latest applications in high-resolution microscopy, live-cell, and single-molecule imaging. We further discuss remaining limitations, which are (i) lower photostabilization of most self-healing dyes when compared to solution additives, (ii) limited mechanistic understanding on the influence of the biochemical environment and molecular oxygen on self-healing, and (iii) the lack of cheap and facile bioconjugation strategies. Finally, we provide ideas on how to further advance self-healing dyes, show new data on redox blinking caused by double-stranded DNA, and highlight forthcoming work on intramolecular photostabilization of fluorescent proteins.
Item Type: | Journal article |
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EU Funded Grant Agreement Number: | 638536 |
EU Projects: | Horizon 2020 > ERC Grants > ERC Starting Grant > ERC Grant 638536: SM-IMPORT - Substrate import at work: single-molecule studies of ABC transporters |
Faculties: | Biology > Department Biology I |
Subjects: | 500 Science > 570 Life sciences; biology |
URN: | urn:nbn:de:bvb:19-epub-77649-9 |
Language: | English |
Item ID: | 77649 |
Date Deposited: | 26. Oct 2021, 17:53 |
Last Modified: | 03. Jan 2022, 18:04 |