Abstract
CO2-reactivity and neurovascular coupling are sequentially lost within the first 24 h after subarachnoid hemorrhage (SAH). Whether and when these impairments recover is not known. Therefore, we investigated the reactivity of pial and intraparenchymal vessels by in vivo two-photon microscopy one month after experimental SAH. C57BL/6 mice were subjected to either sham surgery or SAH by filament perforation. One month later, cerebral blood flow following CO2-challenge and forepaw stimulation was assessed by laser Doppler fluxmetry. Diameters of pial and intraparenchymal arterioles were quantified by in vivo two-photon microscopy. One month after SAH, pial and parenchymal vessels dilated in response to CO2. Neurovascular coupling was almost completely absent after SAH: vessel diameter did not change upon forepaw stimulation compared to a 20% increase in sham-operated mice. The current results demonstrate that neurovascular function differentially recovers after SAH: while CO2-reactivity normalizes within one month after SAH, neurovascular coupling is still absent. These findings show an acute and persistent loss of neurovascular coupling after SAH that may serve as a link between early brain injury and delayed cerebral ischemia, two distinct pathophysiological phenomena after SAH that were so far believed not to be directly related.
Item Type: | Journal article |
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Faculties: | Medicine Medicine > Munich Cluster for Systems Neurology (SyNergy) |
Research Centers: | Graduate School of Systemic Neurosciences (GSN) |
Subjects: | 600 Technology > 610 Medicine and health 500 Science > 500 Science |
URN: | urn:nbn:de:bvb:19-epub-78352-1 |
ISSN: | 0271-678X |
Language: | English |
Item ID: | 78352 |
Date Deposited: | 15. Dec 2021, 14:43 |
Last Modified: | 10. Jun 2024, 11:49 |
DFG: | Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 390857198 |