A conserved filamentous assembly underlies the structure of the meiotic chromosome axis

West, Alan M.; Rosenbereg, Scott C.; Ur, Sarah N.; Lehmern, Madison K.; Ye, Qiaozhen; Hagemann, Götz; Caballero, Iracema; Uson, Isabel; MacQueen, Amy J.; Herzog, Franz und Corbett, Kevin D. (2019): A conserved filamentous assembly underlies the structure of the meiotic chromosome axis. In: eLife, Bd. 8, e40372

Abstract

The meiotic chromosome axis plays key roles in meiotic chromosome organization and recombination, yet the underlying protein components of this structure are highly diverged. Here, we show that 'axis core proteins' from budding yeast (Red1), mammals (SYCP2/SYCP3), and plants (ASY3/ASY4) are evolutionarily related and play equivalent roles in chromosome axis assembly. We first identify 'closure motifs' in each complex that recruit meiotic HORMADs, the master regulators of meiotic recombination. We next find that axis core proteins form homotetrameric (Red1) or heterotetrameric (SYCP2:SYCP3 and ASY3:ASY4) coiled-coil assemblies that further oligomerize into micron-length filaments. Thus, the meiotic chromosome axis core in fungi, mammals, and plants shares a common molecular architecture, and likely also plays conserved roles in meiotic chromosome axis assembly and recombination control.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Chemie und Pharmazie > Department Biochemie
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie
• ISSN: 2050-084X
• Sprache: Englisch