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Kandil, Rima; Xie, Yuran; Mehta, Aditi; Merkel, Olivia (2020): A Method for Targeted Nonviral siRNA Delivery in Cancer and Inflammatory Diseases. In: Jain, Kewal K. (ed.) : Drug Delivery Systems. Methods in molecular biology, Vol. 2059. pp. 155-166
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Small interfering RNA (siRNA)-based therapy has been subject of intense research since the discovery of RNA interference (RNAi), providing a tool to potentially silence any chosen gene. Nevertheless, efficient delivery still presents a major hurdle to translating this promising technology into medical practice. Here, we describe a straightforward method to prepare and characterize an effective delivery system consisting of low-molecular-weight polyethylenimine (PEI) and transferrin (Tf). Tf-PEI polyplexes are not only able to successfully transport and protect the sensitive nucleic acid payload from degradation but also to selectively deliver the siRNA to transferrin receptor (TfR)-overexpressing cells, playing key roles in the pathology of numerous cancer types as well as inflammatory diseases.