Abstract
Small interfering RNA (siRNA)-based therapy has been subject of intense research since the discovery of RNA interference (RNAi), providing a tool to potentially silence any chosen gene. Nevertheless, efficient delivery still presents a major hurdle to translating this promising technology into medical practice. Here, we describe a straightforward method to prepare and characterize an effective delivery system consisting of low-molecular-weight polyethylenimine (PEI) and transferrin (Tf). Tf-PEI polyplexes are not only able to successfully transport and protect the sensitive nucleic acid payload from degradation but also to selectively deliver the siRNA to transferrin receptor (TfR)-overexpressing cells, playing key roles in the pathology of numerous cancer types as well as inflammatory diseases.
Item Type: | Book Section |
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Faculties: | Chemistry and Pharmacy > Department of Pharmacy |
Subjects: | 600 Technology > 610 Medicine and health |
ISBN: | 978-1-4939-9797-8 (print) ; 978-1-4939-9798-5 (online) |
Language: | English |
Item ID: | 84418 |
Date Deposited: | 14. Jan 2022, 11:41 |
Last Modified: | 14. Jan 2022, 11:41 |