A Method for Targeted Nonviral siRNA Delivery in Cancer and Inflammatory Diseases

Kandil, Rima; Xie, Yuran; Mehta, Aditi und Merkel, Olivia (2020): A Method for Targeted Nonviral siRNA Delivery in Cancer and Inflammatory Diseases. In: Jain, Kewal K. (Hrsg.): Drug Delivery Systems. Methods in molecular biology, Bd. 2059. S. 155-166

Abstract

Small interfering RNA (siRNA)-based therapy has been subject of intense research since the discovery of RNA interference (RNAi), providing a tool to potentially silence any chosen gene. Nevertheless, efficient delivery still presents a major hurdle to translating this promising technology into medical practice. Here, we describe a straightforward method to prepare and characterize an effective delivery system consisting of low-molecular-weight polyethylenimine (PEI) and transferrin (Tf). Tf-PEI polyplexes are not only able to successfully transport and protect the sensitive nucleic acid payload from degradation but also to selectively deliver the siRNA to transferrin receptor (TfR)-overexpressing cells, playing key roles in the pathology of numerous cancer types as well as inflammatory diseases.

• Dokumententyp: Buchbeitrag
• Fakultät: Chemie und Pharmazie > Department für Pharmazie - Zentrum für Pharmaforschung
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISBN: 978-1-4939-9797-8 (print) ; 978-1-4939-9798-5 (online)
• Sprache: Englisch