Publikationen von Zakhartchenko, Valeri

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Anzahl der Publikationen: 44

Zeitschriftenartikel

Lange, Andreas; Medugorac, Ivica

; Ali, Asghar; Kessler, Barbara; Kurome, Mayuko; Zakhartchenko, Valeri; Hammer, Sabine E.

; Hauser, Andreas

; Denner, Joachim

; Dobenecker, Britta; Wess, Gerhard; Tan, Paul L. J.; Garkavenko, Olga; Reichart, Bruno

; Wolf, Eckhard

und Kemter, Elisabeth

(2024): Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation. In: Xenotransplantation, Bd. 31, Nr. 2 [PDF, 3MB]

Flisikowska, Tatiana; Egli, Jerome; Flisikowski, Krzysztof; Stumbaum, Marlene; Kueng, Erich; Ebeling, Martin; Schmucki, Roland; Georges, Guy; Singer, Thomas; Kurome, Mayuko; Kessler, Barbara; Zakhartchenko, Valeri; Wolf, Eckhard; Weber, Felix; Schnieke, Angelika und Iglesias, Antonio (2022): A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies. In: Nature Biomedical Engineering, Bd. 6, Nr. 11: S. 1248-1256

Stirm, Michael; Fonteyne, Lina Marie; Shashikadze, Bachuki; Stoeckl, Jan B.; Kurome, Mayuko; Kessler, Barbara; Zakhartchenko, Valeri; Kemter, Elisabeth; Blum, Helmut; Arnold, Georg J.; Matiasek, Kaspar; Wanke, Ruediger; Wurst, Wolfgang; Nagashima, Hiroshi; Knieling, Ferdinand; Walter, Maggie C.; Kupatt, Christian; Froehlich, Thomas; Klymiuk, Nikolai; Blutke, Andreas und Wolf, Eckhard (2022): Pig models for Duchenne muscular dystrophy-from disease mechanisms to validation of new diagnostic and therapeutic concepts. In: Neuromuscular Disorders, Bd. 32, Nr. 7: S. 543-556

Kemter, Elisabeth

; Citro, Antonio; Wolf‐van Buerck, Lelia; Qiu, Yi; Böttcher, Anika; Policardi, Martina; Pellegrini, Silvia; Valla, Libera; Alunni‐Fabbroni, Marianna; Kobolák, Julianna; Kessler, Barbara; Kurome, Mayuko; Zakhartchenko, Valeri; Dinnyes, Andras; Cyran, Clemens C.; Lickert, Heiko; Piemonti, Lorenzo; Seissler, Jochen und Wolf, Eckhard (2022): Transgenic pigs expressing near infrared fluorescent protein—A novel tool for noninvasive imaging of islet xenotransplants. In: Xenotransplantation, Bd. 29, Nr. 1 [PDF, 1MB]

Simmet, Kilian; Kurome, Mayuko; Zakhartchenko, Valeri; Reichenbach, Horst-Dieter; Springer, Claudia; Bahr, Andrea; Blum, Helmut; Philippou-Massier, Julia und Wolf, Eckhard (2022): OCT4/POU5F1 is indispensable for the lineage differentiation of the inner cell mass in bovine embryos. In: Faseb Journal, Bd. 36, Nr. 6 [PDF, 1MB]

; Matiasek, Kaspar; Walter, Maggie C.; Kupatt, Christian; Ziegler, Sibylle; Bartenstein, Peter; Fröhlich, Thomas; Klymiuk, Nikolai; Blutke, Andreas und Wolf, Eckhard (19. November 2021): A scalable, clinically severe pig model for Duchenne muscular dystrophy. In: Disease Models & Mechanisms, Bd. 14, Nr. 12 [PDF, 3MB]

Kalla, Daniela; Flisikowski, Krzysztof; Yang, Kaiyuan; Sanguesa, Laura Beltran; Kurome, Mayuko; Kessler, Barbara; Zakhartchenko, Valeri; Wolf, Eckhard; Lickert, Heiko; Saur, Dieter; Schnieke, Angelika und Flisikowska, Tatiana (8. Oktober 2021): The Missing Link: Cre Pigs for Cancer Research. In: Frontiers in Oncology, Bd. 11, 755746 [PDF, 7MB]

Bebbere, Daniela; Ulbrich, Susanne E.; Giller, Katrin; Zakhartchenko, Valeri; Reichenbach, Horst-Dieter; Reichenbach, Myriam; Verma, Paul J.; Wolf, Eckhard; Ledda, Sergio und Hiendleder, Stefan (14. Mai 2021): Mitochondrial DNA Depletion in Granulosa Cell Derived Nuclear Transfer Tissues. In: Frontiers in Cell and Developmental Biology, Bd. 9, 664099 [PDF, 2MB]

Simmet, Kilian; Wolf, Eckhard und Zakhartchenko, Valeri (2021): Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How. In: International Journal of Molecular Sciences, Bd. 22, Nr. 1, 236

Rieblinger, Beate; Sid, Hicham; Duda, Denise; Bozoglu, Tarik; Klinger, Romina; Schlickenrieder, Antonina; Lengyel, Kamila; Flisikowski, Krzysztof; Flisikowska, Tatiana; Simm, Nina; Grodziecki, Alessandro; Perleberg, Carolin; Bahr, Andrea; Carrier, Lucie; Kurome, Mayuko; Zakhartchenko, Valeri; Kessler, Barbara; Wolf, Eckhard; Kettler, Lutz; Luksch, Harald; Hagag, Ibrahim T.; Wise, Daniel; Kaufman, Jim; Kaufer, Benedikt B.; Kupatt, Christian; Schnieke, Angelika und Schusser, Benjamin (2021): Cas9-expressing chickens and pigs as resources for genome editing in livestock. In: Proceedings of the National Academy of Sciences of the United States of America, Bd. 118, Nr. 10, e2022562118

Springer, Claudia; Zakhartchenko, Valeri; Wolf, Eckhard und Simmet, Kilian (2021): Hypoblast Formation in Bovine Embryos Does Not Depend on NANOG. In: Cells, Bd. 10, Nr. 9, 2232

Stirm, Michael; Fonteyne, Lina Marie; Shashikadze, Bachuki; Lindner, Magdalena; Chirivi, Maila; Lange, Andreas; Kaufhold, Clara; Mayer, Christian; Medugorac, Ivica; Kessler, Barbara; Kurome, Mayuko; Zakhartchenko, Valeri; Hinrichs, Arne; Kemter, Elisabeth; Krause, Sabine; Wanke, Rudiger; Arnold, Georg J.; Wess, Gerhard; Nagashima, Hiroshi; de Angelis, Martin Hrabe; Flenkenthaler, Florian; Kobelke, Levin Arne; Bearzi, Claudia; Rizzi, Roberto; Baehr, Andrea; Reese, Sven; Matiasek, Kaspar; Walter, Maggie C.; Kupatt, Christian; Ziegler, Sibylle; Bartenstein, Peter; Froehlich, Thomas; Klymiuk, Nikolai; Blutke, Andreas und Wolf, Eckhard (2021): A scalable, clinically severe pig model for Duchenne muscular dystrophy. In: Disease Models & Mechanisms, Bd. 14, Nr. 12, dmm049285

Zettler, Silja; Renner, Simone; Kemter, Elisabeth; Hinrichs, Arne; Klymiuk, Nikolai; Backman, Mattias; Riedel, Evamaria Olga; Müller, Christiane; Streckel, Elisabeth; Braun-Reichhart, Christina; Martins, Ana Sofia; Kurome, Mayuko; Kessler, Barbara; Zakhartchenko, Valeri; Flenkenthaler, Florian; Arnold, Georg Josef; Fröhlich, Thomas; Blum, Helmut; Blutke, Andreas; Wanke, Rüdiger und Wolf, Eckhard (2020): A decade of experience with genetically tailored pig models for diabetes and metabolic research. In: Animal Reproduction, Bd. 17, Nr. 3, e20200064

Hinrichs, Arne; Riedel, Evamaria O.; Klymiuk, Nikolai; Blutke, Andreas; Kemter, Elisabeth; Laengin, Matthias; Dahlhoff, Maik; Kessler, Barbara; Kurome, Mayuko; Zakhartchenko, Valeri; Jemiller, Eva-Maria; Ayares, David; Bidlingmaier, Martin; Flenkenthaler, Florian; Hrabe de Angelis, Martin; Arnold, Georg J.; Reichart, Bruno; Fröhlich, Thomas und Wolf, Eckhard (2020): Growth hormone receptor knockout to reduce the size of donor pigs for preclinical xenotransplantation studies. In: Xenotransplantation, Bd. 28, Nr. 2, e12664

Chiumia, Daniel; Schulke, Katy; Groebner, Anna E.; Waldschmitt, Nadine; Reichenbach, Horst-Dieter; Zakhartchenko, Valeri; Bauersachs, Stefan und Ulbrich, Susanne E. (2020): Initiation of Conceptus Elongation Coincides with an Endometrium Basic Fibroblast Growth Factor (FGF2) Protein Increase in Heifers. In: International Journal of Molecular Sciences, Bd. 21, Nr. 5, 1584

Chiumia, Daniel; Hankele, Anna-Katharina; Groebner, Anna E.; Schulke, Katy; Reichenbach, Horst-Dieter; Giller, Katrin; Zakhartchenko, Valeri; Bauersachs, Stefan und Ulbrich, Susanne E. (2020): Vascular Endothelial Growth Factor A and VEGFR-1 Change during Preimplantation in Heifers. In: International Journal of Molecular Sciences, Bd. 21, Nr. 2, 544

Fischer, Konrad; Rieblinger, Beate; Hein, Rabea; Sfriso, Riccardo; Zuber, Julia; Fischer, Andrea; Klinger, Bernhard; Liang, Wei; Flisikowski, Krzysztof; Kurome, Mayuko; Zakhartchenko, Valeri; Kessler, Barbara; Wolf, Eckhard; Rieben, Robert; Schwinzer, Reinhard; Kind, Alexander und Schnieke, Angelika (2020): Viable pigs after simultaneous inactivation of porcine MHC class I and three xenoreactive antigen genes GGTA1, CMAH and B4GALNT2. In: Xenotransplantation, Bd. 27, Nr. 1, e12560

Reichart, Bruno; Langin, Matthias; Radan, Julia; Mokelke, Maren; Buttgereit, Ines; Ying, Jiawei; Fresch, Ann Kathrin; Mayr, Tanja; Issl, Lara; Buchholz, Stefan; Michel, Sebastian; Ellgass, Reinhard; Mihalj, Maks; Egerer, Stefanie; Baehr, Andrea; Kessler, Barbara; Kemter, Elisabeth; Kurome, Mayuko; Zakhartchenko, Valeri; Steen, Stig; Sjoberg, Trygve; Paskevicius, Audrius; Kruger, Luise; Fiebig, Uwe; Denner, Joachim; Godehardt, Antonia W.; Tonjes, Ralf R.; Milusev, Anastasia; Rieben, Robert; Sfriso, Riccardo; Walz, Christoph; Kirchner, Thomas; Ayares, David; Lampe, Karen; Schonmann, Uwe; Hagl, Christian; Wolf, Eckhard; Klymiuk, Nikolai; Abicht, Jan-Michael und Brenner, Paolo (2020): Pig-to-non-human primate heart transplantation: The final step toward clinical xenotransplantation? In: Journal of Heart and Lung Transplantation, Bd. 39, Nr. 8: S. 751-757

Kurome, Mayuko; Baehr, Andrea; Simmet, Kilian; Jemiller, Eva-Maria; Egerer, Stefanie; Dahlhoff, Maik; Zakhartchenko, Valeri; Nagashima, Hiroshi; Klymiuk, Nikolai; Kessler, Barbara und Wolf, Eckhard (2019): Targeting Gal epitopes for multi-species embryo immunosurgery. In: Reproduction Fertility and Development, Bd. 31, Nr. 4: S. 820-826

Lavagi, Ilaria; Krebs, Stefan; Simmet, Kilian; Beck, Andrea; Zakhartchenko, Valeri; Wolf, Eckhard und Blum, Helmut (2018): Single-cell RNA sequencing reveals developmental heterogeneity of blastomeres during major genome activation in bovine embryos. In: Scientific Reports, Bd. 8, 4071 [PDF, 1MB]

Simmet, Kilian; Zakhartchenko, Valeri; Philippou-Massier, Julia; Blum, Helmut; Klymiuk, Nikolai und Wolf, Eckhard (2018): OCT4/POU5F1 is required for NANOG expression in bovine blastocysts. In: Proceedings of the National Academy of Sciences of the United States of America, Bd. 115, Nr. 11: S. 2770-2775

Matsunari, Hitomi; Watanabe, Masahito; Nakano, Kazuaki; Enosawa, Shin; Umeyama, Kazuhiro; Uchikura, Ayuko; Yashima, Sayaka; Fukuda, Toru; Klymiuk, Nikolai; Kurome, Mayuko; Kessler, Barbara; Wünschd, Annegret; Zakhartchenko, Valeri; Wolf, Eckhard; Hanazono, Yutaka; Nagaya, Masaki; Umezawa, Akihiro; Nakauchi, Hiromitsu und Nagashima, Hiroshi (2018): Modeling lethal X-linked genetic disorders in pigs with ensured fertility. In: Proceedings of the National Academy of Sciences of the United States of America, Bd. 115, Nr. 4: S. 708-713

Simmet, Kilian; Zakhartchenko, Valeri und Wolf, Eckhard (2018): Comparative aspects of early lineage specification events in mammalian embryos - insights from reverse genetics studies. In: Cell Cycle, Bd. 17, Nr. 14: S. 1688-1695

Rieblinger, Beate; Fischer, Konrad; Kind, Alexander; Saller, Benedikt S.; Baars, Wiebke; Schuster, Marion; Wolf-van Buerck, Lelia; Schaeffler, Andrea; Flisikowska, Tatiana; Kurome, Mayuko; Zakhartchenko, Valeri; Kessler, Barbara; Flisikowski, Krzysztof; Wolf, Eckhard; Seissler, Jochen; Schwinzer, Reinhard und Schnieke, Angelika (2018): Strong xenoprotective function by single-copy transgenes placed sequentially at a permissive locus. In: Xenotransplantation, Bd. 25, Nr. 2, e12382

Egerer, Stefanie; Fiebig, Uwe; Kessler, Barbara; Zakhartchenko, Valeri; Kurome, Mayuko; Reichart, Bruno; Kupatt, Christian; Klymiuk, Nikolai; Wolf, Eckhard; Denner, Joachim und Bähr, Andrea (2018): Early weaning completely eliminates porcine cytomegalovirus from a newly established pig donor facility for xenotransplantation. In: Xenotransplantation, Bd. 25, Nr. 4, e12449

Kurome, Mayuko; Leuchs, Simon; Kessler, Barbara; Kemter, Elisabeth; Jemiller, Eva-Maria; Foerster, Beatrix; Klymiuk, Nikolai; Zakhartchenko, Valeri und Wolf, Eckhard (2017): Direct introduction of gene constructs into the pronucleus-like structure of cloned embryos: a new strategy for the generation of genetically modified pigs. In: Transgenic Research, Bd. 26, Nr. 2: S. 309-318

Kemter, Elisabeth; Cohrs, Christian M.; Schäfer, Matthias; Schuster, Marion; Steinmeyer, Klaus; Wolf-van Buerck, Lelia; Wolf, Andrea; Wuensch, Annegret; Kurome, Mayuko; Kessler, Barbara; Zakhartchenko, Valeri; Loehn, Matthias; Ivashchenko, Yuri; Seissler, Jochen; Schulte, Anke M.; Speier, Stephan und Wolf, Eckhard (2017): INS-eGFP transgenic pigs: a novel reporter system for studying maturation, growth and vascularisation of neonatal islet-like cell clusters. In: Diabetologia, Bd. 60, Nr. 6: S. 1152-1156

Fischer, Konrad; Kraner-Scheiber, Simone; Petersen, Björn; Rieblinger, Beate; Buermann, Anna; Flisikowska, Tatiana; Flisikowski, Krzysztof; Christan, Susanne; Edlinger, Marlene; Baars, Wiebke; Kurome, Mayuko; Zakhartchenko, Valeri; Kessler, Barbara; Plotzki, Elena; Szczerbal, Izabela; Switonski, Marek; Denner, Joachim; Wolf, Eckhard; Schwinzer, Reinhard; Niemann, Heiner; Kind, Alexander und Schnieke, Angelika (2016): Efficient production of multi-modified pigs for xenotransplantation by 'combineering', gene stacking and gene editing. In: Scientific Reports, Bd. 6, 29081 [PDF, 1MB]

Popken, Jens; Schmid, Volker J.

; Strauss, Axel; Guengoer, Tuna; Wolf, Eckhard und Zakhartchenko, Valeri (2016): Stage-dependent remodeling of the nuclear envelope and lamina during rabbit early embryonic development. In: Journal of Reproduction and Development, Bd. 62, Nr. 2: S. 127-135

Popken, Jens; Graf, Alexander; Krebs, Stefan; Blum, Helmut; Schmid, Volker J.

; Strauss, Axel; Guengoer, Tuna; Zakhartchenko, Valeri; Wolf, Eckhard und Cremer, Thomas (2015): Remodeling of the Nuclear Envelope and Lamina during Bovine Preimplantation Development and Its Functional Implications.
In: PLOS ONE 10(5), e0124619 [PDF, 9MB]

Popken, Jens; Dahlhoff, Maik; Guengoer, Tuna; Wolf, Eckhard und Zakhartchenko, Valeri (2015): 3D structured illumination microscopy of mammalian embryos and spermatozoa. In: BMC Developmental Biology 15:46 [PDF, 2MB]

Popken, Jens; Brero, Alessandro; Koehler, Daniela; Schmid, Volker J.

; Strauss, Axel; Wuensch, Annegret; Guengoer, Tuna; Graf, Alexander; Krebs, Stefan; Blum, Helmut; Zakhartchenko, Valeri; Wolf, Eckhard und Cremer, Thomas (2014): Reprogramming of fibroblast nuclei in cloned bovine embryos involves major structural remodeling with both striking similarities and differences to nuclear phenotypes of in vitro fertilized embryos. In: Nucleus, Bd. 5, Nr. 6: S. 555-589

Popken, Jens; Dahlhoff, Maik; Guengoer, Tuna; Schmid, Volker J.

; Strauss, Axel; Cremer, Thomas; Zakhartchenko, Valeri und Wolf, Eckhard (2014): Nuclear invaginations adapt to rabbit early embryonic developement. In: Reproduction, Fertility and Development, Bd. 27, Nr. 1: S. 139

Li, Shun; Flisikowska, Tatiana; Kurome, Mayuko; Zakhartchenko, Valeri; Kessler, Barbara; Saur, Dieter; Kind, Alexander; Wolf, Eckhard; Flisikowski, Krzysztof und Schnieke, Angelika (2014): Dual Fluorescent Reporter Pig for Cre Recombination: Transgene Placement at the ROSA26 Locus.
In: PLOS ONE 9(7), e102455 [PDF, 960kB]

Kurome, Mayuko; Geistlinger, Ludwig; Kessler, Barbara; Zakhartchenko, Valeri; Klymiuk, Nikolai; Wuensch, Annegret; Richter, Anne; Baehr, Andrea; Kraehe, Katrin; Burkhardt, Katinka; Flisikowski, Krzysztof; Flisikowska, Tatiana; Merkl, Claudia; Landmann, Martina; Durkovic, Marina; Tschukes, Alexander; Kraner, Simone; Schindelhauer, Dirk; Petri, Tobias; Kind, Alexander; Nagashima, Hiroshi; Schnieke, Angelika; Zimmer, Ralf und Wolf, Eckhard (2013): Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set. In: BMC Biotechnology 13:43 [PDF, 617kB]

Popken, J.; Sterr, M.; Markaki, Y.; Cremer, Marion; Beck, A.; Dahlhoff, Maik; Habermann, Felix A.; Fezert, P.; Güngör, Tuna; Reichenbach, M.; Wuensch, Annegret; Wolf, E.; Zakhartchenko, Valeri und Cremer, Thomas (2013): Studies Of Nuclear Architecture In Mammalian Pre-Implantation Embryos And Embryonic Stem Cells Using Super-Resolution Fluorescence Microscopy. In: Reproduction, fertility and development, Bd. 25, Nr. 1: S. 200

Richter, Anne; Kurome, Mayuko; Kessler, Barbara; Zakhartchenko, Valeri; Klymiuk, Nikolai; Nagashima, Hiroshi; Wolf, Eckhard und Wuensch, Annegret (2012): Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig. In: BMC Biotechnology 12:84 [PDF, 1MB]

Leuchs, Simon; Saalfrank, Anja; Merkl, Claudia; Flisikowska, Tatiana; Edlinger, Marlene; Durkovic, Marina; Rezaei, Nousin; Kurome, Mayuko; Zakhartchenko, Valeri; Kessler, Barbara; Flisikowski, Krzysztof; Kind, Alexander; Wolf, Eckhard und Schnieke, Angelika (2012): Inactivation and inducible oncogenic mutation of p53 in gene targeted pigs.
In: PLOS ONE 7(10), e43323 [PDF, 592kB]

de Montera, Béatrice; Zeihery, Dalia E.; Müller, Sigrid; Jammes, Hélène; Brem, Gottfried; Reichenbach, Horst-Dietrich; Scheipl, Fabian; Chavatte-Palmer, Pascale; Zakhartchenko, Valeri; Schmitz, Oliver J.; Wolf, Eckhard; Renard, Jean-Paul und Hiendleder, Stefan (2010): Quantification of Leukocyte Genomic 5-Methylcytosine Levels Reveals Epigenetic Plasticity in Healthy Adult Cloned Cattle. In: Cellular Reprogramming, Bd. 12, Nr. 2: S. 175-181

Lepikhov, Konstantin; Zakhartchenko, Valeri; Hao, R. u.; Yang, Feikun; Wrenzycki, Christine; Niemann, Heiner; Wolf, Eckhard und Walter, Joern (2008): Evidence for conserved DNA and histone H3 methylation reprogramming in mouse, bovine and rabbit zygotes. In: Epigenetics & Chromatin 1:8 [PDF, 2MB]

Wuensch, Annegret; Habermann, Felix A.; Kurosaka, Satoshi; Klose, Regina; Zakhartchenko, Valeri; Reichenbach, Horst-Dieter; Sinowatz, Fred; McLaughlin, K. J. und Wolf, Eckhard (2007): Quantitative monitoring of pluripotency gene activation after somatic cloning in cattle. In: Biology of Reproduction, Bd. 76, Nr. 6: S. 983-991 [PDF, 409kB]

Konferenzbeitrag

Matthiesen, Marieke; Reichenbach, Horst-Dieter; Habermann, Felix A.; Zakhartchenko, Valeri; Reichenbach, M.; Arnold, Georg J.; Demant, M. und Wolf, E. (2010): Maturation and developmental competence of bovine oocytes at different life cycle stages: Ovum Pick Up and functional in vitro studies - preliminary results. 43. Jahrestagung Physiologie u. Pathologie der Fortpflanzung, gleichzeitig 35. Veterinär-Humanmedizinische Gemeinschaftstagung, München, Deutschland, 24. – 26. Februar 2010. Reproduction in domestic animals / Supplement. Bd. 45, Nr. 1 S. 31-32

Habermann, Felix A.; Matthiesen, Marieke; Reichenbach, Horst-Dieter; Zakhartchenko, Valeri; Reichenbach, M.; Arnold, Georg J.; Demant, M. und Wolf, E. (2010): In vitro studies on the developmental competence of oocytes from heifers, young and old cows. 43. Jahrestagung Physiologie u. Pathologie der Fortpflanzung, gleichzeitig 35. Veterinär-Humanmedizinische Gemeinschaftstagung, München, Deutschland, 24. – 26. Februar 2010. Reproduction in domestic animals / Supplement. Bd. 45, Nr. 1 S. 17-18

Matthiesen, Marieke; Arnold, Georg J.; Demant, M.; Zakhartchenko, Valeri; Habermann, Felix A.; Reichenbach, Horst-Dieter; Reichenbach, M. und Wolf, E. (2009): Developmental competence of bovine oocytes obtained from animals at different ages: structural, molecular and functional analyses - preliminary results. 42. Jahrestagung über Physiologie und Pathologie der Fortpflanzung, gleichzeitig 34. Veterinär-humanmedizinische Gemeinschaftstagung, Leipzig, Deutschland, 26. - 27. Februar 2009. Reproduction in domestic animals / Supplement. Bd. 44, Nr. 1 S. 23-24

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