Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release

Lau, Benjamin; Beine-Golovchuk, Olga; Kornprobst, Markus; Cheng, Jingdong; Kressler, Dieter; Jady, Beata; Kiss, Tamas; Beckmann, Roland und Hurt, Ed (2022): Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release. In: Cell Reports, Vol. 41, No. 8, 111684

Abstract

Ribosome synthesis begins in the nucleolus with 90S pre-ribosome construction, but little is known about how the many different snoRNAs that modify the pre-rRNA are timely guided to their target sites. Here, we report a role for Cms1 in such a process. Initially, we discovered CMS1 as a null suppressor of a nop14 mutant impaired in Rrp12-Enp1 factor recruitment to the 90S. Further investigations detected Cms1 at the 18S rRNA 3' major domain of an early 90S that carried H/ACA snR83, which is known to guide pseudouridylation at two target sites within the same subdomain. Cms1 co-precipitates with many 90S factors, but Rrp12-Enp1 encir-cling the 3' major domain in the mature 90S is decreased. We suggest that Cms1 associates with the 3' major domain during early 90S biogenesis to restrict premature Rrp12-Enp1 binding but allows snR83 to timely perform its modification role before the next 90S assembly steps coupled with Cms1 release take place.

• Item Type: Journal article
• Faculties: Chemistry and Pharmacy > Department of Pharmacy; Chemistry and Pharmacy > Department of Biochemistry
• Subjects: 500 Science > 540 Chemistry
• ISSN: 2211-1247
• Language: English