Single-Cell Radiotracer Allocation via Immunomagnetic Sorting to Disentangle PET Signals at Cellular Resolution

Bartos, Laura M.; Kunte, Sebastian T.; Beumers, Philipp; Xiang, Xianyuan; Wind, Karin; Ziegler, Sibylle; Bartenstein, Peter; Choi, Hongyoon; Lee, Dong Soo; Haass, Christian; Baumgarten, Louisa von; Tahirovic, Sabina; Albert, Nathalie L. ; Lindner, Simon and Brendel, Matthias (2022): Single-Cell Radiotracer Allocation via Immunomagnetic Sorting to Disentangle PET Signals at Cellular Resolution. In: Journal of Nuclear Medicine, Vol. 63, No. 10: pp. 1459-1462

Abstract

With great interest, our independent groups of scientists located in Korea and Germany recognized the use of a very similar methodologic approach to quantify the uptake of radioactive glucose (18F-FDG) at the cellular level. The focus of our investigations was to disentangle micro-glial 18F-FDG uptake. To do so, CD11b immunomagnetic cell sorting was applied to isolate microglia cells after in vivo 18F-FDG injection, to allow simple quantification via a g-counter. Importantly, this technique reveals a snapshot of cellular glucose uptake in living mice at the time of injection since 18F-FDG is trapped by hexokinase phosphorylation without a further opportunity to be metabolized. Both studies indicated high 18F-FDG uptake of single CD11b-positive microglia cells and a significant increase in microglial 18F-FDG uptake when this cell type is activated in the presence of amyloid pathology. Furthermore, another study noticed that immunomagnetic cell sorting after tracer injection facilitated determination of high 18F-FDG uptake in myeloid cells in a range of tumor models. Here, we aim to discuss the rationale for single -cell radiotracer allocation via immunomagnetic cell sorting (scRadiotrac-ing) by providing examples of promising applications of this innovative technology in neuroscience, oncology, and radiochemistry.

• Item Type: Journal article
• Form of publication: Publisher's Version
• Faculties: Medicine; Medicine > Munich Cluster for Systems Neurology (SyNergy)
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 0161-5505
• Language: English
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 421887978
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 390857198