Extracellular vesicle-mediated trafficking of molecular cues during human brain development

Forero, Andrea; Pipicelli, Fabrizia; Moser, Sylvain; Baumann, Natalia; Grätz, Christian; Gonzalez Pisfil, Mariano; Pfaffl, Michael W. ; Pütz, Benno; Kielkowski, Pavel ; Cernilogar, Filippo M.; Maccarrone, Giuseppina; Giaimo, Rossella Di und Cappello, Silvia (2024): Extracellular vesicle-mediated trafficking of molecular cues during human brain development. In: Cell Reports, Bd. 43, Nr. 10, 114755

Abstract

Cellular crosstalk is an essential process influenced by numerous factors, including secreted vesicles that transfer nucleic acids, lipids, and proteins between cells. Extracellular vesicles (EVs) have been the center of many studies focusing on neurodegenerative disorders, but whether EVs display cell-type-specific features for cellular crosstalk during neurodevelopment is unknown. Here, using human-induced pluripotent stem cell-derived cerebral organoids, neural progenitors, neurons, and astrocytes, we identify heterogeneity in EV protein content and dynamics in a cell-type-specific and time-dependent manner. Our results support the trafficking of key molecules via EVs in neurodevelopment, such as the transcription factor YAP1, and their localization to differing cell compartments depending on the EV recipient cell type. This study sheds new light on the biology of EVs during human brain development.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin ; Chemie und Pharmazie > Department Chemie ; Medizin > Physiologisches Institut ; Medizin > Adolf-Butenandt-Institut ; Medizin > Munich Cluster for Systems Neurology (SyNergy) ; Medizin > Klinikum der LMU München > Medizinische Klinik und Poliklinik I (Kardiologie)
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 2211-1247
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 390857198