Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures

Hammelmann, Verena; Stieglitz, Marc Sebastian; Huelle, Henrik; Le Meur, Karim; Kass, Jennifer; Bruemmer, Manuela; Gruner, Christian; Roetzer, Rene Dominik; Fenske, Stefanie; Hartmann, Jana; Zott, Benedikt; Luthi, Anita; Spahn, Saskia; Moser, Markus; Isbrandt, Dirk; Ludwig, Andreas; Konnerth, Arthur; Wahl-Schott, Christian und Biel, Martin (2019): Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures. In: Jci Insight, Bd. 4, Nr. 9, e126418

Abstract

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are dually gated channels that are operated by voltage and by neurotransmitters via the cAMP system. cAMP-dependent HCN regulation has been proposed to play a key role in regulating circuit behavior in the thalamus. By analyzing a knockin mouse model (HCN2EA), in which binding of cAMP to HCN2 was abolished by 2 amino acid exchanges (R591E, T592A), we found that cAMP gating of HCN2 is essential for regulating the transition between the burst and tonic modes of firing in thalamic dorsal-lateral geniculate (dLGN) and ventrobasal (VB) nuclei. HCN2EA mice display impaired visual learning, generalized seizures of thalamic origin, and altered NREM sleep properties. VB-specific deletion of HCN2, but not of HCN4, also induced these generalized seizures of the absence type, corroborating a key role of HCN2 in this particular nucleus for controlling consciousness. Together, our data define distinct pathological phenotypes resulting from the loss of cAMP-mediated gating of a neuronal HCN channel.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Chemie und Pharmazie > Department für Pharmazie - Zentrum für Pharmaforschung ; Medizin > Munich Cluster for Systems Neurology (SyNergy)
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie
• Sprache: Englisch
• DFG: Gefördert durch die Deutsche Forschungsgemeinschaft (DFG) - 390857198